Biodegradation of Petroleum Degrading Microorganism Present in Soil
DOI:
https://doi.org/10.68050/JAMS.2026.669Keywords:
BIODEGRADATION OF PETROLEUM DEGRADINGAbstract
In this present study, the soil sample was collected from various petrol bunks
around Trichy and Tanjore. Bacterial and fungal species were isolated from the soil
sample and they were identified. Among this bacterial isolates, two dominant species,
Bacillus subtilis and Pseudomonas aeruginosa were selected for petroleum
biodegradation and one dominant fungal species, Aspergillus niger was also selected for
biodegradation. The degradation ability was screened based on the soil
characteristics. After treatment all the parameters were analysed which was compared
with initial values. All the values were reduced after bacterial and fungal treatment.
Among this highest degradation was noted in bacteria than in fungi. The plasmid
was isolated from the isolated bacteria, Pseudomonas aeruginosa. Also to study the
protein nature SDS PAGE was also performed.In 1979, for the first time Anand mohan
Chakrabarthy, an India borne American scientist (at the time with general electric Co
USA) obtained a strain of Pseudomonas putida that contained the XYL and NAH
plasmid as well as a hybrid plasmid derived by recombinating parts of CAM and OCT
(these are . The bacteria laden straw can be stored until required. When the straw was
spread over oil slicks, the straw soaked up the oil and bacteria broke up the oil into non
polluting and harmless products. Finally conclude that the isolated bacterial strains
efficiently degrade the petroleum contaminated soil, so that bacterial strains such as
Bacillus subtilis and Pseudomonas aeruginosa were recommended for petroleum
biodegradation.
References
1. Atlas, R.M. and Bartha, R. 1972. Biodegradation of petroleum in seawater at lower temperature. Can.J.Micrbiol., (18) 1851 – 1855.
2. Balba, M..T., Al-Awadhi N., Al-Daher R .,1998. Bioremediation of oil-contaminated soil: microbiological methods for feasibility assessment and field evaluation. Journal of Microbiological Methods., Volume 32, Issue 2, Pages 155–164.
3. Bantley.J.P., “Principles of Measurement Systems”, Longman, Singapore, 1998 Bot.Res.Intl., 2(4):248 – 252.
4. Bens.E.M.,and Soli, G. 1973. Selective substrate utilization by marine hydrocarbon clastic bacteria. Biotechnol.Bio.Eng. 15:285 – 297.
5. Bhattacharya.D., Krishnan.S., Lal.B.,and Sarma, P.M., 2004. Degradation of polycyclic aromatic hydrocarbon by a newly discovered enteric bacterium, Leclercia adecarboxylata. J. Appl. Environ. Microbiol., 70: 3163-3166.
6. Bodour, A.A., and Miller Maler ,R.M., 1998., Application of a modified drop- collapse technique for surfactant quantification and screning of biosurfactanttt producing microorganisms, Journal of microbiological method 32, 273 – 280.
7. Bushnell,L.D., and Hass, H.E.,1941. Growth of Pseudomonas sp., on petroleum., J. Bacteriology.41 :653- 667.
8. Chakrabarthy, A.M. 1976., Plasmid on Pseudomonas., Annu Rev. Genet 10., 7 – 30.
9. Danielle M. Pala., Denize D. de Carvalho José Carlos Pinto. Geraldo., L. Sant Anna Jr., 2006. A suitable model to describe bioremediation of a petroleum-contaminated soil international Biodeterioration & Biodegradation Volume 58, Issues 3–4 , , Pages 254–260
10. Danelle Mohammed, Adesh Ramsubhag and Denise M., 2007. Beckles An Assessment of the Biodegradation of Petroleum Hydrocarbons in Contaminated Soil Using Non-indigenous, Commercial Microbes Earth and Environmental Science Water, Air, & Soil Pollution Volume 182, Numbers 1-4, 349-356
11. Dave, H ., Ramakrishna C Bhatt Desai J. D 2007. Biodegradation of slop oil from a petrochemical industry and bioreclamation of slop oil contaminated soil World Journal of Microbiology and Biotechnology Volume 10 PAGES 653-656.
12. Euisang Lee., and Banks M. K., 1993. Bioremediation of petroleum contaminated soil using vegetation. A microbial studyJournal of Environmental Science and Health ., Part A: Environmental Science and Engineering and Toxicology Volume 28, Issue 10 pages 2187-2198.
13. Fan, C.Y.; Krishnamurthy, S. 1995[Environmental Protection Agency, Edison, NJ (United States)] Enzyumes for enhancing bioremediation of petroleum-contaminated soils. A brief review Journal of the Air and Waste Management Association; Journal Volume: 45; Journal Issue: 6: pp. 453-460.
14. Gupta, R.K. Gaur, M. Lowry, D.P. Jaroli and U.K. Chauhan, 2011. Bioremediation of Petroleum oil Contaminated Soil and Water. Research Journal of Environmental Toxicology, 5: 1-26
15. Grażyna Płaza., Grzegorz Nałęcz-Jawecki., Krzysztof Ulfig., Robin L. Brigmon 2005 The application of bioas says as indicators of petroleum-contaminated soil remediation Volume 59, Issue 2,, Pages 289–296
16. Ghosal, D., Chatterjee. I., and Chakrabarthy, A.M 1985., Concepts of biodegradation. J. science., 228: 135 – 142.
17. Inga Sarand., Sari Timonen., Eeva-Liisa Nurmiaho-Lassila., Teija Koivula Kielo Haahtela., Martin Romantschuk., Robin Sen1998*. Microbial biofilms and catabolic plasmid harbouring degradative fluorescent pseudomonads in Scots pine mycorrhizospheres developed on petroleum contaminated soil FEMS Microbiology EcologyVolume 27, Issue 2, pages 115–126.
18. Lonsane,B.K., Singh,H.D., and Barauh,J.N 1975., Codification of petroleum hydrocarbon utilising microorganism. Journal. Scient Ind. Res. 34 : 215 – 230.
19. Lin, S.1996. Biosurfactant. Recent reviews., J chem. Biotechnology 66 : 109 -120.
20. Michael H. Huesemann 1994 . Guidelines for land treating petroleum hydrocarbon‐contaminated soils Journal of Soil Contamination., Volume 3, Issue 3, pages 299-318.
21. Okerentugba P.O and Ezeronye O.U 2003 Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluent in Nigeria African Journal of Biotechnology Vol. 2 (9), pp. 288-292
22. Nilanjana Das., Preethy Chandran 2010. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview Biotechnology research international (2010) Volume: 2011 , Pages: 941810
23. Owen Ward, Ajay Singh and J. Van Hamme 2001 Accelerated biodegradation of petroleum hydrocarbon waste Biomedical and Life Sciences Journal of Industrial Microbiology & Biotechnology Volume 30 PAGES 324 – 332
24. Pui-Yi Cheung and Brian K. Kinkle 2001. Mycobacterium Diversity and Pyrene Mineralization in Petroleum-Contaminated Soils Appl. Environ. Microbiol. vol. 67 no. 5 2222-2229.
25. Robert A. Kanaly., Shigeaki Harayama., 2000. Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria J. Bacteriol. vol. 182 no. 8 2059-2067
26. Sobral.L.G.,and Trindade, P.V.O. 2005. Bioremediation of a weathered and a recently oil contaminated soils from Brazil: a comparison study. Chemosphere, 58: 515 – 522
27. Thayumanavan, Rahman Laks, K.S.M. and hmanaperumalsamy, P. 2001. Biodegradation of petroleum refinery waste oil sludge, Poll.Res., 20(2): 156- 161.
28. WalwortJ.L,. Woolard C.R,. BraddockJ.F and. ReynoldsC.M.,1995 Enhancement and inhibition of soil petroleum biodegradation through the use of fertilizer nitrogen: An approach to determining optimum levels Journal of Soil Contamination Volume 6, Issue 5 pages 465-480
29. Walker, J.D. Colwell, R.R and Petrakis, L.1975. Evaluation of petroleum degrading potential of bacteria from water and sediment. Appl.Microbiol., 30: 1036- 1039.
30. Whyte, L .G ., Bourbonniére, L., Greer C .W., 1997. Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane (alk) and naphthalene (nah) catabolic pathways., Appl. Environ. Microbiol. vol. 63 no. 9 3719-372.3
31. Zulfiqar Ali Malik and Safia Ahmed 2012 Degradation of petroleum hydrocarbons by oil field isolated bacterial consortium African Journal of Biotechnology Vol. 11 (3), pp. 650-658.
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